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(A) Schematic illustration on the WT1 exons and localization from the primers made use of for semi-quantitative RT-PCR (arrows) are revealed. (B) Ex4a(+)WT1 mRNA expression was determined by RT CR applying 4a-F and Ex6-R primer pair that amplifies only Ex4a(+)WT1 isoform in 6 various WT1-expressing cancer cells (AZ-521, HT-1080, LU99B, K562, Kasumi-1 and HL60) and just one WT1-expressiong normal k