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Following, the ratio of Ex4a(+)WT1 isoform to 17AA(+) WT1 isoform was resolute by RT-PCR working with Ex4-forward and Ex6-reverse primer pair in WT1-expressing most cancers cell strains (LU99B and K562) and identified to get about 1/2 and 1/4 in LU99B and K562 cancer cells, respectively (Fig 4D). These results indicated the Ex4a(+)WT1 isoform was expressed as a small isoform along with the major W