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Fects of chronic Zn deficiency on the gut microbial composition. EarlyFects of chronic Zn deficiency on the gut microbial composition. Early work by Smith et al. [15] elucidated a role of the host microbiota in Zn homeostasis, whereby conventionally-raised (CR, Conventionally-raised) mice required nearly twice as much dietary Zn than did their germ-free (GF) counterparts. In the same study,