(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms Immense Wise
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(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms was resolute by RT CR making use of Ex4-F and Ex6-R primer pair that amplifies both equally Ex4a(+)WT1 and key WT1 isoforms in two distinct WT1-expressing most cancers cells (LU99B and K562). (B-D) Actin is made use of being an inside manage. Results are consultant of a few independent experiments.(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms
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