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(A) Schematic representation of the WT1 exons and localization in the primers utilized for semi-quantitative RT-PCR (arrows) are shown. (B) Ex4a(+)WT1 mRNA expression was resolute by RT CR employing 4a-F and Ex6-R primer pair that amplifies only Ex4a(+)WT1 isoform in six various WT1-expressing cancer cells (AZ-521, HT-1080, LU99B, K562, Kasumi-1 and HL60) and just one WT1-expressiong regular kidne