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(A) Schematic illustration on the WT1 exons and localization of your primers applied for semi-quantitative RT-PCR (arrows) are demonstrated. (B) Ex4a(+)WT1 mRNA expression was firm by RT CR using 4a-F and Ex6-R primer pair that amplifies only Ex4a(+)WT1 isoform in 6 unique WT1-expressing cancer cells (AZ-521, HT-1080, LU99B, K562, Kasumi-1 and HL60) and 1 WT1-expressiong usual kidney cells 293. (C