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In this experiment Vapyrin RNAi-2 and GUS RNAi transgenic roots arising from single transformation events were sampled, and in each case part of the root was stained to visualize the fungus, whereas the remainder was used for transcript analysis by qRT-PCR. Vapyrin transcript levels were reduced significantly (60-fold) in the single Vapyrin RNAi-2 roots relative to the GUS RNAi roots (Figure?2b).
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